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5x phusion reaction buffer  (New England Biolabs)


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    Structured Review

    New England Biolabs 5x phusion reaction buffer
    5x Phusion Reaction Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1641 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/5x+phusion+hf+buffer/Phusion+HF+Buffer/pmc13018877-52-0-5
    Average 96 stars, based on 1641 article reviews
    5x phusion reaction buffer - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Incubation:

    Article Title: The earliest changes in the translatome upon human T cell activation
    Article Snippet: The sequences were purchased from IDT as ssDNA oligonucleotide templates which underwent anneal and fill-in reactions to form dsDNA templates for in-vitro transcription. .. Using separate reactions, 5X Phusion HF Buffer (NEB) was added to 10 μM of each ssDNA oligo and 10 μM of a scaffold anti-sense primer (purchased from IDT, 5’-AAAAGCACCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCCTTATTTTAACTTGCTATTTCTAGCTCTAAAAC-3’) and incubation consisted of denaturation at 95°C for 5 min, annealing from 95°C to 50°C at 0.1°C/sec, hold at 50°C for 5 mins, then 50°C to 37°C at 0.1°C/sec. .. Following annealing, 10 mM dNTPs (NEB), 2U Phusion HF DNA Polymerase (NEB) and nuclease-free water were added to a final volume of 50μl.

    Polymerase Chain Reaction:

    Article Title: Characterization of variants associated with Cerebral Small Vessel Disease identifies a functional SNV in Versican
    Article Snippet: Table 3) was synthesized containing 102 bp of sequence upstream and downstream of the variant, the major or minor variant, a multi-enzyme cloning site, a 10 bp barcode and a reverse adaptor (Twist Bioscience South San Francisco, CA). .. To amplify the library, 12 independent PCR reactions were set up containing 1 ng the synthesized MPRA oligos, 10 μl of 5X Phusion HF buffer, 200 μM dNTPs, 1-unit Phusion HF DNA polymerase (New England Biolabs), 0.5uM MPRA oligo-f and MPRA oligo-r (Supp. ..

    Article Title: Polyacrylamide bead split-pool method for microbial community analysis
    Article Snippet: .. Per reaction, the PCR reactions consisted of 4μl of 5x Phusion HF buffer (NEB), 0.4 μL of 10mM dNTP Solution Mix (NEB), 1 μL of each 10μM primer / primer mix, 0.2 μL of 2,000 units/mL Phusion® Hot Start Flex DNA Polymerase (NEB) and 13.4 μl of template DNA (1st PCR) or 10μl of template DNA and 3.4μl of H 2 O (2nd PCR). ..

    Article Title: Polyacrylamide bead split-pool method for microbial community analysis
    Article Snippet: .. Per reaction, the PCR reactions consisted of 4 μL of 5x Phusion HF buffer (NEB), 0.4 μL of 10mM dNTP Solution Mix (NEB), 1 μL of 10 μM forward (i5) and reverse (i7) primers each, 0.2 μL of 2,000 units/mL Phusion® Hot Start Flex DNA Polymerase (NEB), 11.4 μL of sterile H 2 O and 2μl of the template DNA. ..

    Article Title: A Preliminary Study on Identifying the Predator Community of Invasive Bactericera cockerelli (Hemiptera: Triozidae) and Developing Molecular Identification Tools for Testing Field Predation.
    Article Snippet: .. PCR amplification was performed using nuclease-free biotechnology-grade water (Fisher Biotech, Wembley, WA, Australia), 5X Phusion HF buffer, 10 mM dNTPs, Phusion DNA polymerase (New England Biolabs, Ipswich, MA, USA), 1.25 μL of each primer and 1 μL of insect DNA in a 25 μL reaction. .. PCR was performed in a Veriti Thermal Cycler (Thermo Fisher Scientific Inc., Waltham, MA, USA) or an Eppendorf® Mastercycler (Sigma-Aldrich, St. Louis, MI, USA).

    Synthesized:

    Article Title: Characterization of variants associated with Cerebral Small Vessel Disease identifies a functional SNV in Versican
    Article Snippet: Table 3) was synthesized containing 102 bp of sequence upstream and downstream of the variant, the major or minor variant, a multi-enzyme cloning site, a 10 bp barcode and a reverse adaptor (Twist Bioscience South San Francisco, CA). .. To amplify the library, 12 independent PCR reactions were set up containing 1 ng the synthesized MPRA oligos, 10 μl of 5X Phusion HF buffer, 200 μM dNTPs, 1-unit Phusion HF DNA polymerase (New England Biolabs), 0.5uM MPRA oligo-f and MPRA oligo-r (Supp. ..

    other:

    Article Title:
    Article Snippet: After confirmation of successful amplification, the PCR product was purified 355 using AMPure XP (Beckman-Coulter) and subsequently quantified using Nanodrop 356 (Thermo Fisher Scientific).

    Sterility:

    Article Title: Polyacrylamide bead split-pool method for microbial community analysis
    Article Snippet: .. Per reaction, the PCR reactions consisted of 4 μL of 5x Phusion HF buffer (NEB), 0.4 μL of 10mM dNTP Solution Mix (NEB), 1 μL of 10 μM forward (i5) and reverse (i7) primers each, 0.2 μL of 2,000 units/mL Phusion® Hot Start Flex DNA Polymerase (NEB), 11.4 μL of sterile H 2 O and 2μl of the template DNA. ..

    Amplification:

    Article Title: A Preliminary Study on Identifying the Predator Community of Invasive Bactericera cockerelli (Hemiptera: Triozidae) and Developing Molecular Identification Tools for Testing Field Predation.
    Article Snippet: .. PCR amplification was performed using nuclease-free biotechnology-grade water (Fisher Biotech, Wembley, WA, Australia), 5X Phusion HF buffer, 10 mM dNTPs, Phusion DNA polymerase (New England Biolabs, Ipswich, MA, USA), 1.25 μL of each primer and 1 μL of insect DNA in a 25 μL reaction. .. PCR was performed in a Veriti Thermal Cycler (Thermo Fisher Scientific Inc., Waltham, MA, USA) or an Eppendorf® Mastercycler (Sigma-Aldrich, St. Louis, MI, USA).



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